Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (MALS verified)

• Catalog Number: orb1818945
• Category: Proteins
• Description: Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
• Reactivity: Human
• Tag: C-Fc | C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 50.2 kDa & 63.1 kDa
• Target: IL-4 R alpha & IL-13 R alpha 1
• Source: Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (orb1818945) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & NP_001551.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag with a linear range of 0.6-5 ng/mL (QC tested).
• Expression Region: Met 26 - His 232 & Gly 22 - Thr 343
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH 7.4
• Note: For research use only
• Application notes: Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 50.2 kDa & 63.1 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag & Fc Tag (Cat. No. orb1818945) at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag (Cat. No. orb1149283) with a linear range of 0.6-5 ng/mL (QC tested).