Human IL-1RA

SKU: orb3002383

Description

Recombinant Human Interleukin-1 Receptor Antagonist Protein is produced by our E.coli expression system and the target gene encoding Arg26-Glu177 is expressed.

Key Properties

• Expression System: E.coli
• Biological Activity: Cell Based Assay: Measured by its ability to inhibit IL-1 beta induced NF-kB signaling in 293-IL1 Res cells(Cat#Xorb3002421).The ED50 for this effect is 2-12 ng/mL.（QC Verified）
• Tag: Tag free
• Molecular Weight: Predicted: 17.26 KDa. Observed: 18 KDa, reducing conditions
• Expression Region: Arg26-Glu177
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 7.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Interleukin-1 Receptor Antagonist Protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 Inhibitor; Anakinra; IL1RN; IL1F3; IL1RA

Quick Database Links

UniProt

P18510