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Human IL-12B Protein, His Tag

Catalog Number: orb257709

DispatchUsually dispatched within 5-10 working days
$ 380.00
Catalog Numberorb257709
CategoryProteins
DescriptionHuman IL-12B, His Tag is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1).
TargetIL-12B
ConjugationUnconjugated
TagC-10×His
ReactivityHuman
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Purity95%
Protein SequenceAAH67499.1
UniProt IDP29460
MW36.6 kDa
Application notesThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.6 kDa. The protein migrates as 44 kDa and 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression SystemHEK293
Endotoxins1.0 EU per μg
Biological OriginHuman
Biological ActivityLoaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. ) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Expression RegionIle 23 - Ser 328
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
NoteFor research use only
NCBIAAH67499.1
Human IL-12B Protein, His Tag

SDS-PAGE analysis of Human IL-12B protein

Human IL-12B Protein, His Tag

Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Human IL12A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743293]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.

    Mammalian

    50 μg, 10 μg, 100 μg
  • Human IL23A and IL12B Heterodimer Protein, hFc Tag and His Tag [orb1743335]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation.

    E.coli

    10 μg, 50 μg, 100 μg
  • Human IL12B Protein, His Tag [orb1290821]

    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation.

    Mammalian

    100 μg, 10 μg, 50 μg
  • IL-12B Protein, Human, Recombinant (His) [orb1958570]

    97.10%

    36.2 kDa (predicted); 40-50 kDa (reducing condition, due to glycosylation)

    100 μg, 200 μg, 500 μg
  • Recombinant Human IL-12B/P40 Protein with His tag, His Tag [orb1550693]

    > 95% by SDS-PAGE.

    Recombinant Human IL-12B/P40 Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ile23-Ser328) of human IL-12B/P40 (Accession #NP_002178.2) fused with a 6��His Tag at the C-terminus.

    100 μg, 50 μg