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Catalog Number | orb1290821 |
---|---|
Category | Proteins |
Description | Recombinant human IL12B protein with C-terminal 6×His tag |
Reactivity | Mouse |
Tag | C-6×His Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation. |
Target | IL12B |
UniProt ID | P29460 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | IL-12B;CLMF p40;IL-12 subunit p40;NKSF2 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Mouse TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.
Unconjugated | |
95% | |
36.6 kDa | |
Human IL-12B, His Tag (orb257709) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # P29460-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation. | |
E.coli |
98.00% | |
39.7 kDa & 27.2 kDa (predicted) |
> 90%, determined by SDS-PAGE | |
This protein contains the human IL12A (NP_0008732)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B (NP_0057462)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified and expressed from HEK293 Cells. |