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Catalog Number | orb1290821 |
---|---|
Category | Proteins |
Description | Recombinant human IL12B protein with C-terminal 6×His tag |
Target | IL12B |
Tag | C-6×His Tag |
Reactivity | Mouse |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
UniProt ID | P29460 |
MW | The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation. |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | IL-12B;CLMF p40;IL-12 subunit p40;NKSF2 |
Note | For research use only |
Mouse TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.8 and 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL23A-hFc and IL12B-His is approximately 35-55 kDa due to glycosylation. | |
E.coli |
> 90%, determined by SDS-PAGE | |
This protein contains the human IL12A (NP_0008732)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; This protein contains the human IL27B (NP_0057462)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified and expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the human IL12 complex composed of IL12B (NP_0021782) ( Met1-Ser328) and the mature form of human IL12A ( NP_0008732)( Arg 23 - Ser 219 ) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 90%, determined by SDS-PAGE | |
This protein contains the human IL23 complex composed of IL12B (NP_0021782)(Met1-Ser328) and the mature form of human IL23A (NP_0576681) (Arg20-Pro189) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |