Human IgG IgA IgM (H&L) Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347196
• Category: Antibodies
• Description: Human IgG IgA IgM (H&L) antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: AP
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum. This reagent is suitable for the detection of all human immunoglobulin isotypes, subclasses and chain combinations.
• Immunogen: Human IgG, IgA and IgM whole molecule
• Tested applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:3,000
• Application notes: Anti-Human IgG IgA IgM Alkaline Phosphatase conjugate has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: Goat Anti-Human IgG IgA IgM Antibody alkaline phos
• Note: For research use only

Dot Blot of Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Human IgG Row 2 - Human IgA Row 3 - Human IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Human IgG IgA IgM Antibody Alkaline Phosphatase Conjugated at 1:1000 for 1 HR at RT. Block: orb348637 for 1 HR at RT.