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Human IgG (H&L) Antibody Peroxidase Conjugated

Catalog Number: orb347262

DispatchUsually dispatched within 5-10 working days
$ 480.00
Catalog Numberorb347262
CategoryAntibodies
DescriptionHuman IgG (H&L) antibody (Peroxidase)
ClonalityPolyclonal
Species/HostMouse
IsotypeIgG
ConjugationHRP
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
PurityThis product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Mouse Serum, Human IgG and Human Serum.
ImmunogenHuman IgG whole molecule
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Application notesAnti-Human IgG Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesmouse anti-Human IgG peroxidase conjugated Antibod
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NoteFor research use only
Human IgG (H&L) Antibody Peroxidase Conjugated

ELISA of purified Mouse Anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG HRP. Each well was coated in duplicate with 1.0 µg of Human IgG (p/n orb346219). The working dilution is 1:28000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).