Human IgG (H&L) Antibody

SKU: orb346652

Description

Human IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 10.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Human Antibody, Rb-a-Human IgG, Human Antibody in Rabbit, Human Secondary Antibody

ELISA results using Rabbit Anti-Human IgG. Impact of behavioral variables on M. leprae infection levels. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibody levels in children and adolescents were measured by ELISA, using either protein A, anti- IgM or anti-IgG to detect responses. In a, samples were stratified by recorded knowledge of eating armadillo meat as either yes (n = 14) or no (n = 64). In b, samples were stratified by recorded knowledge of BCG re-vaccination following identification of the index leprosy case as either yes (n = 54) or no (n = 16). Data are displayed as box and whisker plots, with the box representing the Q1 to Q3 interquartile range and the horizontal bar representing the median of the optical density of the samples. Individual dots indicate outliers, and p-values are indicated by the lines above each indicated group.

ELISA results using Rabbit Anti-Human IgG. Influence of index case on M. leprae infection levels. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibody levels in children and adolescents were measured by ELISA, using either protein A, anti- IgM or anti-IgG to detect responses. In a, samples were stratified by reported WHO operational classification of the index case as either MB (n = 66) or PB (n = 16). In b, samples were stratified by estimated duration of exposure to the index leprosy case as either less than 10 years (n = 45) or greater than 10 years (n = 37). Data are displayed as box and whisker plots, with the box representing the Q1 to Q3 interquartile range and the horizontal bar representing the median of the optical density of the samples. Individual dots indicate outliers, and p-values are indicated by the lines above each indicated group.