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Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Catalog Number: orb347340

DispatchUsually dispatched within 5-10 working days
$ 440.00
Catalog Numberorb347340
CategoryAntibodies
DescriptionHuman IgG (H&L) antibody (Biotin)
ClonalityPolyclonal
Species/HostDonkey
IsotypeIgG
ConjugationBiotin
ReactivityHuman
Form/AppearanceLyophilized
Concentration1.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
ImmunogenHuman IgG whole molecule
Tested applicationsELISA, IHC, WB
Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application notesAnti-Human IgG (H&L) Biotin Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 is suggested for this product.
Antibody TypeSecondary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesDonkey Anti Human IgG Biotin Conjugated Antibody,
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NoteFor research use only