Human IgG gamma Antibody Alkaline Phosphatase

• Catalog Number: orb347239
• Category: Antibodies
• Description: HUMAN IgG gamma antibody (Alkaline Phosphatase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: AP
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Human IgG (gamma chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), Human IgG, Human serum and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.
• Immunogen: Human IgG gamma heavy chain
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4,000 - 1:16,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application notes: Anti-Human IgG Alkaline Phosphatase has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Alternative names: goat anti-Human IgG (gamma chain) alkaline phospha
• Note: For research use only

Western Blot of Alkaline Phosphatase Conjugated Goat anti-Human IgG Gamma Chain antibody. Lane 1: Human IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase human secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 55 kDa for Human IgG Gamma Chain. Other band(s): none.