Human IgG Fc Antibody

• Catalog Number: orb347275
• Category: Antibodies
• Description: Human IgG F(c) antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 5.014 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
• Immunogen: Anti-Human IgG F(c) fragment was produced by repeated immunization with Human IgG F(c) fragment in rabbit.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:26,000-1:46,000, IHC: 1:1,000 - 1:6,000, WB: 1:3,000 - 1:30,000
• Application notes: Anti-Human IgG F(c) fragment has been tested by western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Human IgG F(c) Antibody, Human IgG Fc Antibody, Rb
• Note: For research use only

Western Blot of Rabbit anti-Human Fc antibody. Lane 1: Human Fc (p/n orb346220). Lane 2: none. Load: 100 ng per lane. Primary antibody: Human Fc antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human Fc. Other band(s): Human Fc splice variants and isoforms.