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Human IgG F(ab')2 Antibody pre-absorbed
Catalog Number: orb347280
| Catalog Number | orb347280 |
|---|---|
| Category | Antibodies |
| Description | Human IgG F(ab')2 antibody |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid (sterile filtered) |
| Concentration | 2.0mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Purity | Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins. |
| Immunogen | Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment. |
| Tested applications | DOT, ELISA, IHC, WB |
| Dilution range | ELISA: 1:350,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000 |
| Application notes | Anti-Human IgG F(ab')2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Antibody Type | Secondary Antibody |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Alternative names | F(ab')2 Human IgG Antibody, Rb-a-Human F(ab')2 IgG Read more... |
| Note | For research use only |
ELISA Results of Rabbit Anti-Human IgG F(ab')2 Antibody (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab')2 Mx Ms. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab')2 (p/n orb346221) [Red Line], Human IgG (p/n orb346219) [Green Line], and Mouse IgG (p/n orb2652749) [Blue Line]. The working dilution of Human IgG F(ab')2 MX is 1:56300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).
