Human IgG F(ab')2 Antibody pre-absorbed

SKU: orb347280

Description

Human IgG F(ab')2 antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution range: ELISA: 1:350,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG F(ab')2 antibody has been tested by ELISA and dot blot and is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Human IgG F(ab')2 fragment was produced in rabbit by repeated immunization with human IgG F(ab')2 fragment.
• Purity: Anti-Human IgG F(ab')2 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 2.0mg/mL
• Disclaimer: For research use only

Alternative Names

F(ab')2 Human IgG Antibody, Rb-a-Human F(ab')2 IgG, F(ab')2 Human IgG Antibody in Rabbit, Pre-Adsorbed Human IgG Secondary Antibody

ELISA Results of Rabbit Anti-Human IgG F(ab')2 Antibody (Min X Mouse Serum Proteins) tested against purified Human IgG F(ab')2 Mx Ms. Each well was coated in duplicate with 1.0 µg of Human IgG F(ab')2 (p/n orb346221) [Red Line], Human IgG (p/n orb346219) [Green Line], and Mouse IgG (p/n orb2652749) [Blue Line]. The working dilution of Human IgG F(ab')2 MX is 1:56300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).