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Catalog Number | orb611008 |
---|---|
Category | Proteins |
Description | Recombinant Human IGF1 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | IGF1 A70T Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB and 150 mM NaCl, pH 6.0. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKST |
Application notes | Cytokines And Growth Factors |
Source | Escherichia Coli |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IGF1 A70T in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles |
Alternative names | Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano Read more... |
Note | For research use only |
Greater than 95% as determined by reducing SDS-PAGE. | |
7.3 KDa | |
Mammalian |
ELISA, FA, HPLC, SDS-PAGE, WB | |
Unconjugated | |
> 95% pure by SDS-PAGE and HPLC analyses. | |
9.1 kDa |
Greater than 90% as determined by SDS-PAGE. | |
34.7 kDa | |
E.coli |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 110-120 (Alpha subunit) and 52-55 (Beta subunit) kDa based on Tris-Bis PAGE result. |