Human IgA (alpha chain) Antibody Fluorescein Conjugated

SKU: orb347285

Description

Human IgA (alpha chain) antibody (FITC)

Images & Validation

• Tested Applications: DOT, FC, FLISA, IF
• Dilution Range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgA Fluorescein Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 2.77 moles Fluorescein (FITC) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 1.0 mL

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgA alpha heavy chain
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against Human IgG or Human IgM. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Human IgA (alpha chain) fluorescein conjugated Antibody, rabbit anti-Human IgA alpha Antibody FITC conjugation

Dot Blot of Human IgA (alpha chain) Antibody Fluorescein Conjugated. Antigen: Human IgA. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Human IgA (alpha chain) Antibody Fluorescein Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 60 min at RT.