Recombinant Human IFN-beta Protein

SKU: orb3002500

Description

Recombinant Human Interferon beta is produced by our Mammalian expression system and the target gene encoding Met22-Asn187 is expressed with a human IgG1 Fc tag at the N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Activity: ELISA: Immobilized Recombinant Human IFNAR2 (C-Fc)(Cat#orb2994011) at 2μg/ml (100 μl/well) can bind Recombinant Human IFNB1*. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human IFNB1 is 4.36ng/ml.(Regularly tested).
• Tag: N-Fc
• Molecular Weight: Predicted: 46.2kDa. Observed: 47-57kDa, reducing conditions
• Expression Region: Met22-Asn187
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: ＜0.01EU/μg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM NaAC-HAC, 10%Terhalose, 50mM NaCl, 3%Mannitol, 0.01%Tween20, pH 5.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interferon beta; IFN-beta; Fibroblast interferon; IFNB1; IFB; IFNB

Quick Database Links

UniProt

P01574