You have no items in your shopping cart.
Description
Research Area
Infectious Disease & Virology
Images & Validation
−
Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human IFNAR2-Fc(Cat#orb2994011) on Protein A Biosensor, can bind Human IFN alpha2b(Cat#orb3002431) with an affinity constant of 2.98 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED50 for this effect is 5 ng/mL. |
| Tag | Tag free |
| Expression Region | Cys24-Glu188 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 19.4 KDa. Observed: 17 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified). SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2
Similar Products
−- Item 1 of 1
Human IFN alpha2b Protein [orb1471757]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
19.4 KDa
Mammalian
10 μg, 50 μg Human IFNA2 protein [orb391883]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE.
E. coli
50 μg, 10 μg, 500 μgHuman CLEC2B Protein [orb611127]
Greater than 90.0% as determined by SDS-PAGE.
Sf9, Baculovirus cells
1 μg, 5 μg, 50 μg
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
Available Sizes
Select a size below
Usually dispatched within 5-10 working days