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Catalog Number | orb391883 |
---|---|
Category | Proteins |
Description | Recombinant human IFNA2 protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 166 amino acids with a molecular mass of 19400 Daltons. The Interferon-alpha2b gene was obtained from human leukocytes. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE. |
Target | IFNA2 |
Entrez | 3440 |
UniProt ID | P01563 |
Protein Sequence | MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Interferon alpha-2; IFN-alpha-2; Interferon alpha- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
19.2 kDa | |
Yeast |
> 96% as determined by SDS-PAGE and HPLC. | |
19.4 kDa | |
E.Coli |
> 98% as determined by SDS-PAGE and HPLC. | |
19.3 kDa | |
Yeast |
Greater than 95% as determined by SDS-PAGE. | |
19.4 kDa | |
E.coli |