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Description
Research Area
Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways
Images & Validation
−
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E.Coli |
|---|---|
| Reactivity | Human |
| Tag | N-terminal His Tag |
| Expression Region | 1-215aa |
| MW | 24.8 KDa |
| Purity | Greater than 95% by SDS-PAGE gel analyses |
Storage & Handling
−| Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
|---|---|
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, HMGB1, SBP-1, Sulfoglucuronyl carbohydRate binding protein
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Human HMGB1 Protein, His Tag [orb2321419]
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 25.7 kDa after removal of the signal peptide. The apparent molecular mass of HMGB1-His is approximately 15-35 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg Recombinant Human HMGB1 Protein, N-His [orb2969334]
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>95% as determined by SDS-PAGE.
27.02 kDa
1 mg, 50 μg, 100 μgRecombinant Human HMGB1 Protein, N-His [orb2969335]
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27.06 kDa
1 mg, 50 μg, 100 μgRecombinant Human HMGB1 Protein, N-His [orb2836537]
>90% as determined by SDS-PAGE.
26.97 kDa
20 μg, 50 μg, 100 μgRecombinant Human HMGB1 Protein, N-His [orb2836538]
>90% as determined by SDS-PAGE.
27.2 kDa
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
Select a size below
Usually dispatched within 5-10 working days