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Catalog Number | orb2321419 |
---|---|
Category | Proteins |
Description | Recombinant human HMGB1 Protein with C-terminal 6×His tag |
Reactivity | Human |
Tag | C-6×His tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 25.7 kDa after removal of the signal peptide. The apparent molecular mass of HMGB1-His is approximately 15-35 kDa due to glycosylation. |
Target | HMGB1 |
UniProt ID | P09429 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | HMG1; HMG3; HMG-1; SBP-1 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human DSG2 Protein, His Tag on SDS-PAGE under reducing condition.
98.00% | |
13.6 kDa (predicted); 15 kDa (reducing conditions) |
SDS-PAGE: 98.6%; SEC-HPLC: 97.3% | |
26.3 kDa (predicted); 30-34 kDa (reducing condition, due to glycosylation) |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human HMGB1 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Met1-Glu215) of Human HMGB1 fused with a His Tag at the C-terminal. |
> 93%, determined by SDS-PAGE | |
This protein contains the human HMGB1 protein (NP_002119.1) (Met 1-Glu 215) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus and expressed from HEK293 Cells. |