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Description
Research Area
Cancer Biology
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | BLI: Loaded Recombinant Human HGF (C-6His) (Cat#orb3002483) on HIS1K Biosensor, can bind Ficlatuzumab with an affinity constant of 5.50 pM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human HGF (C-6His) (Cat#orb3002483) on HIS1K Biosensor, can bind Rilotumumab with an affinity constant of 6.66 pM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.3-1.5 ng/ml (QC verified). Mouse bile duct organoids were cultured with EGF (Cat#orb3002493) , R-spondin 1 (Cat#orb3002497) ,FGF-10 (Cat#orb3002478) and HGF (Cat#orb3002483). The organoids showed good morphology. |
| Tag | C-6His |
| Expression Region | Gln32-Ser728 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 26&53.7 KDa. Observed: 32-38&50-65 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris，500mM NaCl，3%Trehalose，0.02% Tween 80，pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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