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Recombinant Human HGF R Protein
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human HGF-6His (Cat#orb3002483) at 10μg/ml (100 μl/well) can bind Human HGF R-His* (Cat#orb2994182).*: Biotinylated by NHS-biotin prior to testing The ED50 of Recombinant HGF R-His* (Cat#orb2994182) is 9.47 ug/ml |
| Tag | C-6His |
| Molecular Weight | Predicted: 102.5 KDa. Observed: 130&80-95&40-50 KDa, reducing conditions |
| Expression Region | Glu25-Thr932 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human HGF R Protein (Biotin) [orb2993795]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 104.4 KDa. Observed: 75-95&40-50 KDa, reducing conditions
20 μg, 100 μgRecombinant Human HGF R Protein [orb2994269]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 56.9 KDa. Observed: 32&35-57 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human HGF R Protein [orb2994181]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 128.4 KDa. Observed: 170&100-130&45-50 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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Protocol Information
Recombinant Human HGF R Protein (orb2994182)
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