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Catalog Number | orb638748 |
---|---|
Category | Proteins |
Description | Human GUCY2C, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 47.9 kDa |
Target | GUCY2C |
UniProt ID | P25092 |
Protein Sequence | NP_004954.2 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ser 24 - Gln 430 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_004954.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation. | |
Mammalian |
98.00% | |
47.78 kDa (predicted); 72.11 kDa (reducing conditions) |
98.00% | |
65-110 KDa (reducing condition) |
98.00% | |
48.8 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human Guanylyl Cyclase C/GUCY2C Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser24-Gln430) of Human Guanylyl Cyclase C/GUCY2C fused with His Tag and Avi tag at the C-terminal. |