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Catalog Number | orb1173928 |
---|---|
Category | Proteins |
Description | Recombinant human GUCY2C protein with C-terminal 6×His tag |
Reactivity | Human |
Tag | C-6×His Tag |
Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation. |
Target | GUCY2C |
UniProt ID | P25092 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
Alternative names | DIAR6;GC-C;GUC2C;MECIL;MUCIL;STAR Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Human CLEC12A Protein, His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CLEC12A Protein, His Tag (orb689436) can bind Anti-CLEC12A antibody, Rabbit mAb in a linear range of 0.32–1.60 ng/ml.
98.00% | |
47.78 kDa (predicted); 72.11 kDa (reducing conditions) |
98.00% | |
65-110 KDa (reducing condition) |
98.00% | |
48.8 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human Guanylyl Cyclase C/GUCY2C Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser24-Gln430) of Human Guanylyl Cyclase C/GUCY2C fused with His Tag and Avi tag at the C-terminal. |