GPC3 Human Monoclonal Antibody

SKU: orb2958607

Description

Anti-Human GPC3 Antibody (BAR9) is a human monoclonal antibody detecting GPC3 in ELISA, FCM, WB. Suitable for Human and Mouse. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BAR9
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: orb2958607) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human GPC3 antibody. GPC3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # orb2958607, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human GPC3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # orb2958607, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human GPC3 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC3 antibody monoclonal antibody (Catalog # orb2958607, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human GPC3 Antibody.

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1 µg/mL on various samples. Lane 1: HepG2 cell lysate.

Western blot analysis was performed using anti-GPC3/Glypican 3 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant mouse GPC3/Glypican 3 (Catalog No: orb2964901), Lane 2: negative control.

Quick Database Links

UniProt

P51654