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Catalog Number | orb391794 |
---|---|
Category | Proteins |
Description | Recombinant human GFRA1 protein is produced by our Mammalian expression system and the target gene encoding Asp25-Lys429 is expressed with a 6His tag at the C-terminus. |
Target | GFRA1 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGASS HITTKVDHHH HHH |
UniProt ID | P56159 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | GDNFRA; RETL1; TRNR1; GDNF family receptor alpha-1 Read more... |
Note | For research use only |
Entrez | 2674 |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 98%, determined by SDS-PAGE | |
This protein contains the human GFRα1 without the propeptide (NP_6657361) (Met 1-Ser 424) was fused with the a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 80% by SDS-PAGE. | |
Recombinant Human GFRA1/GFR��1/GDNFRA Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala19-Lys429) of human GFRA1/GFR��1/GDNFRA (Accession #NP_665736) fused with an Fc, 6��His Tag at the C-terminus. |