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Catalog Number | orb257477 |
---|---|
Category | Proteins |
Description | Human Flt-3 Ligand, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1). |
Target | Flt-3 Ligand |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_001450.2 |
UniProt ID | P49771 |
MW | 19.9 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 28-30 kDa and 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human Flt-3, Fc Tag (Cat. No. FL3-H5258) at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag (Cat. No. ) with a linear range of 0.1-3 ng/mL (QC tested). |
Expression Region | Thr 27 - Pro 185 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001450.2 |
SDS-PAGE analysis of Human Flt-3 Ligand protein
Immobilized Human Flt-3, Fc Tag (Cat. No. orb570275) at 2 μg/mL (100 μL/well) can bind Human Flt-3 Ligand, His Tag (Cat. No. orb257477) with a linear range of 0.1-3 ng/mL (QC tested).