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Catalog Number | orb545725 |
---|---|
Category | Proteins |
Description | Mouse Flt-3 Ligand, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Arg 188 (Accession # P49772-1). |
Target | Flt-3 Ligand |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_038548.3 |
UniProt ID | P49772 |
MW | 20.2 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Human Flt-3, Fc Tag (Cat. No. FL3-H5258) at 5 μg/mL (100 μL/well) can bind Mouse Flt-3 Ligand, His Tag (Cat. No. ) with a linear range of 0.1-0.6 ng/mL (QC tested). |
Expression Region | Gly 27 - Arg 188 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_038548.3 |
Immobilized Human Flt-3, Fc Tag (Cat. No. orb570275) at 5 μg/mL (100 μL/well) can bind Mouse Flt-3 Ligand, His Tag (Cat. No. orb545725) with a linear range of 0.1-0.6 ng/mL (QC tested).
> 85% by SDS-PAGE. | |
Recombinant Mouse Flt-3 Ligand Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-Arg188) of mouse Flt-3 Ligand (Accession #NP_038548.3.) fused with an Fc, 6��His Tag at the C-terminus. |