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Catalog Number | orb391736 |
---|---|
Category | Proteins |
Description | Recombinant human FGF8 protein is produced by our E. coli expression system and the target gene encoding Gln23-Arg244 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | FGF8 |
Entrez | 2253 |
UniProt ID | P55075 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MQEGPGRGPA LGRELASLFR AGREPQGVSQ QVTVQSSPNF TQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE FLNYPPFTRS LRGSQRTWAP EPR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, pH 7.4. |
Alternative names | AIGF; Fibroblast growth factor 8; FGF-8; Androgen- Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 95% as determined by SDS-PAGE. | |
27.7 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 97% by SDS-PAGE and HPLC analyses | |
28.1kDa |
Greater than 95% as determined by SDS-PAGE. | |
22.5 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human FGF8(Gln23-Arg215) was fused without Tag and expressed in E. coli. |
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