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Human FGF8 protein

SKU: orb391736

Description

Recombinant human FGF8 protein is produced by our E. coli expression system and the target gene encoding Gln23-Arg244 is expressed with a 6His tag at the N-terminus.

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetFGF8
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMGSSHHHHHH SSGLVPRGSH MQEGPGRGPA LGRELASLFR AGREPQGVSQ QVTVQSSPNF TQHVREQSLV TDQLSRRLIR TYQLYSRTSG KHVQVLANKR INAMAEDGDP FAKLIVETDT FGSRVRVRGA ETGLYICMNK KGKLIAKSNG KGKDCVFTEI VLENNYTALQ NAKYEGWYMA FTRKGRPRKG SKTRQHQREV HFMKRLPRGH HTTEQSLRFE FLNYPPFTRS LRGSQRTWAP EPR

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM EDTA, 1mM DTT, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

AIGF; Fibroblast growth factor 8; FGF-8; Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human FGF8 protein (orb391736)

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Available Sizes

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10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 3,100.00
DispatchUsually dispatched within 5-10 working days
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