Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

SKU: orb775974

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epidermal Growth Factor Receptor(EGFR).

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.18 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ErbB-1 ELISA Kit, ErbB1 ELISA Kit, ER1 ELISA Kit, ERB-B1 ELISA Kit, MENA ELISA Kit, HER1 ELISA Kit, Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian ELISA Kit, Proto-oncogene c-ErbB-1 ELISA Kit, Receptor tyrosine-protein kinase erbB-1 ELISA Kit

Quick Database Links

UniProt

P00533