Human Epidermal Growth Factor Receptor (EGFR) EasyStep ELISA Kit

• Catalog Number: orb2302962
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 10 ng/mL
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 25 pg/mL
• Sample Types: Serum, plasma
• Alternative names: ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythrobla
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only
• Expiration Date: Please enquire.