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Description
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | E. coli - derived recombinant Human DSC2 (Arg136-Cys471). |
| Purification | Purified by antigen affinity column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
| Concentration | Lot dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting DSC2 (orb2952741) at a concentration of 93.35 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of DSC2 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
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Protocol Information
DSC2 Rabbit Polyclonal Antibody (orb2952741)
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