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| Catalog Number | orb257395 |
|---|---|
| Category | Proteins |
| Description | Human Dkk-1 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
| Target | Dkk-1 |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | NP_036374.1 |
| UniProt ID | O94907 |
| MW | 26.6 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human Dkk-1 Protein, His Tag, premium grade (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.01-0.156 μg/mL (QC tested). |
| Expression Region | Thr 32 - His 266 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_036374.1 |
Li, Chunyan et al. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Biomed Res Int, 2015, 345413 (2015)
SDS-PAGE analysis of Human Dkk-1 protein
Immobilized Human Dkk-1 Protein, His Tag, premium grade (Cat. No. orb257395) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. orb570288) with a linear range of 0.01-0.156 μg/mL (QC tested).
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Human DKK1 Protein, His Tag [orb1743317]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 26.6 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-His is approximately 35-55 kDa due to glycosylation.
E.coli
10 μg, 50 μg, 100 μg - Item 1 of 1
- Item 1 of 1
Recombinant Human DKK1 Protein, His Tag [orb1550987]
> 95% by SDS-PAGE.
Recombinant Human DKK1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr32-His Tag266) of human DKK1 (Accession #O94907) fused with an 8��His Tag at the N-terminus.
50 μg
