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Catalog Number | orb1743317 |
---|---|
Category | Proteins |
Description | Recombinant Human DKK1 Protein with C-terminal 6×His tag |
Target | DKK1 |
Tag | C-6×His Tag |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM DTT, pH 7.4. |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
UniProt ID | O94907 |
MW | The protein has a predicted molecular mass of 26.6 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-His is approximately 35-55 kDa due to glycosylation. |
Source | E.coli |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | DKK-1;SK |
Note | For research use only |
Greater than 95% as determined by reducing SDS-PAGE.
> 95% by SDS-PAGE. | |
Recombinant Human DKK1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Thr32-His Tag266) of human DKK1 (Accession #O94907) fused with an 8��His Tag at the N-terminus. |
> 90%, determined by SDS-PAGE | |
This protein contains the human DKK1 precursor (NP_0363741) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |
96.70% | |
27.22 kDa (predicted); 42.9 kDa (reducing condition, due to glycosylation) |