CTSB Mouse Monoclonal Antibody

SKU: orb2957425

Description

CTSB Mouse Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in WB application. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Images & Validation

• Tested Applications: WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: B4C4
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: orb2957425, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CTSB Antibody.

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CTSB (Catalog No: orb2961892), 50 ng, Lane 2: recombinant human CTSB (Catalog No: orb2961892), 30 ng, Lane 3: recombinant human CTSB (Catalog No: orb2961892), 20 ng.

Quick Database Links

UniProt

P07858