CTSB Mouse Monoclonal Antibody

SKU: orb2957425

Description

Anti-Human CTSB Antibody (B4C4) is a mouse monoclonal antibody detecting APP secretase in WB. Suitable for Human.

Images & Validation

• Tested Applications: WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: B4C4
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: orb2957425, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CTSB Antibody.

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CTSB (Catalog No: orb2961892), 50 ng, Lane 2: recombinant human CTSB (Catalog No: orb2961892), 30 ng, Lane 3: recombinant human CTSB (Catalog No: orb2961892), 20 ng.

Quick Database Links

UniProt

P07858