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Catalog Number | orb391533 |
---|---|
Category | Proteins |
Description | Recombinant human CSF1 protein is produced by our Mammalian expression system and the target gene encoding Glu33-Arg255 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | CSF1 |
Entrez | 1435 |
UniProt ID | P09603 |
Protein Sequence | EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPRVDHHHHH H |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Macrophage colony-stimulating factor 1; CSF-1; M-C Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
18.4 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
26.17 kDa | |
Mammalian cell |
Unconjugated | |
95% | |
20.3 kDa | |
Human IL-37b, His Tag (orb257616) is expressed from E. coli cells. It contains AA Val 46 - Asp 218 (Accession # AAH20637.1). |
Unconjugated | |
95% | |
26.0 kDa | |
Human M-CSF, His Tag (orb402822) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1). |
Unconjugated | |
95% | |
20.1 kDa | |
Human IL-37, His Tag (orb1146980) is expressed from E. coli cells. It contains AA Lys 27 - Asp 192 (Accession # Q9NZH6-2). |
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