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Catalog Number | orb1087565 |
---|---|
Category | Proteins |
Description | Human Complement C3, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1). |
Target | Complement C3 |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_000055.2 |
UniProt ID | P01024 |
MW | 186.8 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 186.8 kDa. The protein migrates as 80 kDa and 120-130 kDa and 200 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human Complement C3, His Tag (Cat. No. ) at 1 μg/mL (100 μL/well) can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested). |
Expression Region | Ser 23 - Asn 1663 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000055.2 |
Immobilized Human Complement C3, His Tag (Cat. No. orb1087565) at 1 μg/mL (100 μL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).
Unconjugated | |
95% | |
71.3 kDa (β chain) and 113.0 kDa (α chain) |
98.00% | |
17.2 kDa (predicted) |