Cynomolgus Complement C3 Protein, His Tag

SKU: orb1101181

Description

Cynomolgus Complement C3, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asn 1663 (Accession # G7PYU9-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement component 3 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 71.3 kDa (β chain) and 113.0 kDa (α chain). The protein migrates as 64-68 kDa (β chain) and 100-120 kDa (α chain) and 160-180 kDa (intact Complement component 3) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Activity: Immobilized Cynomolgus Complement C3, His Tag at 1 µg/mL (100 µL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested).
• Target: Complement C3
• Reactivity: Monkey
• Tag: C-10×His
• Molecular Weight: 71.3 kDa (β chain) and 113.0 kDa (α chain)
• Expression Region: Thr 23 - Asn 1663
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Cynomolgus Complement C3, His Tag (Cat. No. orb1101181) at 1 μg/mL (100 μL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C3

UniProt

G7PYU9

RefSeq:G7PYU9