Human CD86/B7-2 Antibody

SKU: orb3149055

Description

Anti-Human CD86/B7-2 Antibody (B5F3) is a mouse monoclonal antibody detecting T-lymphocyte activation antigen CD86 in ELISA, FCM, IP. Suitable for Human and Pig. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IP
• Reactivity: Human, Porcine

Key Properties

• Host: Mouse
• Clone No.: B5F3
• Purity: >95% as determined by SDS-PAGE.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD86, CD28LG2, B7-2

Flow-cytometry using anti-human CD86 antibody. Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 monoclonal antibody (Catalog orb3149055, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-CD86/B7-2 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CD86/B7-2 (Catalog No: orb2968560), Lane 2: negative control.

Quick Database Links

UniProt

P42081, K7GMU1