CD81 Human Monoclonal Antibody

SKU: orb2956674

Description

Anti-Human CD81 Antibody (B227XE23) is a human monoclonal antibody detecting CD81 antigen in ELISA, FCM, IHC, WB. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B227XE23
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1

Detects CD81 in indirect ELISAs.

Flow-cytometry using anti-human CD81 antibody. Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog # orb2956674, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CD81 Antibody.

Western blot analysis was performed using anti-CD81 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CD81 (Catalog No: orb2968279), Lane 2: negative control.

Quick Database Links

UniProt

P60033