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| Catalog Number | orb624103 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Membrane cofactor protein protein |
| Tag | C-terminal hFc-tagged |
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
| Purity | Greater than 92% as determined by SDS-PAGE. |
| Protein Sequence | CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY |
| Protein Length | Partial |
| UniProt ID | P15529 |
| MW | 61.6 kDa |
| Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
| Source | Mammalian cell |
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml. |
| Expression Region | 35-328aa |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Alternative names | TLX (Trophoblast leukocyte common antigen) (CD46) |
| Research Area | Cancer Biology |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 μg/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml.

The purity of Human CD46 was greater than 95% as determined by SEC-HPLC
Human | |
7.82-500pg/mL | |
4.69 pg/mL |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 33.83 KDa. Observed: 50-65 KDa, reducing conditions |
ELISA, IHC, WB | |
Human | |
Monoclonal | |
Unconjugated |
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