Human Membrane Cofactor Protein (MCP) ELISA Kit

SKU: orb775700

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Cofactor Protein(MCP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, urine, saliva and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 12.5-800 pg/mL
• Sensitivity: 4.9 pg/mL
• Concentration: 800 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD46 ELISA Kit, MIC10 ELISA Kit, TLX ELISA Kit, TRA2 ELISA Kit, Trophoblast leukocyte common antigen ELISA Kit, CD46 Molecule,Complement Regulatory Protein ELISA Kit, CD46 Molecule,Complement Regulatory protein ELISA Kit

Quick Database Links

UniProt

P15529