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Catalog Number | orb705340 |
---|---|
Category | Proteins |
Description | Recombinant Human CD40 ligand(CD40LG),partial |
Tag | N-terminal hFc-Flag-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 87% as determined by SDS-PAGE. |
MW | 44.5 kDa |
UniProt ID | P29965 |
Protein Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
Protein Length | Partial |
Source | Mammalian cell |
Expression System | 113-261aa |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 μg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/ml. |
Expression Region | 13-261aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | (CD40-L)(T-cell antigen Gp39)(NF-related activatio Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 95% as determined by SDS-PAGE. | |
16.2 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
50.8 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
26.1 kDa | |
Yeast |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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