Human CD34 Mouse Monoclonal Antibody

SKU: orb526618

Description

Human CD34 Mouse Monoclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cell Markers, Developmental Biology, Hematopoietic Stem Cell Markers, Innate Immunity, Molecular Biology, Myeloid Cells, Neuroscience, Stem Cell Research, Surface Molecules, Tumor Biomarkers, cardiovascular Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=0.5ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 4H11
• Immunogen: KLH conjugated synthetic peptide derived from human CD34
• Target: CD34
• Molecular Weight: 105-120 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4).
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.

Blank control: TF-1. Primary Antibody (green line): Mouse Anti-human CD34 antibody (orb526618), dilution: 0.5 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (human CD34) Monoclonal Antibody, Unconjugated (orb526618) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD34

UniProt

P28906