CD326/EPCAM Human Monoclonal Antibody

SKU: orb2975121

Description

Anti-Human CD326/EPCAM Antibody (BOV423) is a human monoclonal antibody detecting Epithelial glycoprotein 314 in FCM. Suitable for Human. Highlights Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: BOV423
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Epithelial glycoprotein 314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, EGP314, TACSTD1, Adenocarcinoma-associated antigen, TROP1, EPCAM, Epithelial glycoprotein, KS 1/4 antigen, Tumor-associated calcium signal transducer 1, M4S1, CD326, Ep-CAM, EGP, Epithelial cell adhesion molecule, M1S2, KSA, Cell surface glycoprotein Trop-1, GA733-2, Epithelial cell surface antigen, MIC18

Flow-cytometry using anti-human CD326 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD326 monoclonal antibody (Catalog: orb2975121) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD326 antibody. CD326 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 antibody monoclonal antibody (Catalog # orb2975121, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD326 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 antibody monoclonal antibody (Catalog # orb2975121, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CD326/EPCAM Antibody.

Quick Database Links

UniProt

P16422