CD274/PD-L1/B7-H1 Mouse Monoclonal Antibody

SKU: orb2975158

Description

Anti-Human CD274/PD-L1/B7-H1 Antibody (B44E5) is a mouse monoclonal antibody detecting B7-H1 in FCM. Suitable for Human. Highlights Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B44E5
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7-H1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, PDCD1L1, B7 homolog 1, PDCD1LG1, PDL1, hPD-L1, Programmed death ligand 1, B7H1, PD-L1, CD274

Detects CD274/PD-L1/B7-H1 in indirect ELISAs.

Flow-cytometry using anti-human CD274 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD274 monoclonal antibody (Catalog: orb2975158) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

Q9NZQ7