Human CCR4 protein

• Catalog Number: orb595152
• Category: Proteins
• Description: Recombinant Human C-C chemokine receptor type 4(CCR4)
• Tag: N-terminal 10xHis-tagged
• Form/Appearance: Liquid or Lyophilized powder
• Purity: Greater than 85% as determined by SDS-PAGE.
• MW: 44.9 kDa
• UniProt ID: P51679
• Protein Sequence: MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL
• Protein Length: Full Length
• Source: in vitro E.coli expression system
• Biological Origin: Homo sapiens (Human)
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/mL can bind Anti-CCR4 recombinant antibody, the EC50 is 7.818-21.25 ng/mL.
• Expression Region: 1-360aa
• Endotoxins: Not test.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Buffer/Preservatives: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Alternative names: K5-5 CD_antigen, CD194
• Note: For research use only
• Application notes: Full Length
• Expiration Date: 6 months from date of receipt.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/ml can bind Anti-CCR4 recombinant antibody. The EC50 is 2.803-3.369 ng/mL.

Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody with an affinity constant of 3.16 nM as detected by SPR Assay (Biacore T200).

Human CCR4 Monoclonal Antibody captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12) (WeSPRTM200).