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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 4
| Application Notes |
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Key Properties
−| Source | in vitro E.coli expression system |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | ①Measured by its binding ability in a functional ELISA. Immobilized Human CCR4 at 5 μg/mL can bind Anti-CCR4 recombinant antibody. The EC50 is 2.803-3.369 ng/mL. ②Human CCR4 Full Length Protein captured on CM5 chip can bind Human CCR4 Monoclonal Antibody with an affinity constant of 3.16 nM as detected by SPR Assay(Biacore T200). ③Human CCR4 Monoclonal Antibody captured on Protein A Chip can bind Human CCR4 Full Length Protein with an affinity constant of 1.71 nM as detected by MetaSPR Assay (in presence of FOS-12). |
| Tag | N-terminal 10xHis-tagged |
| Expression Region | 1-360aa |
| Protein Length | Full Length |
| MW | 44.2 kDa |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Protein Sequence | MNPTDIADTTLDESIYSNYYLYESIPKPCTKEGIKAFGELFLPPLYSLVFVFGLLGNSVVVLVLFKYKRLRSMTDVYLLNLAISDLLFVFSLPFWGYYAADQWVFGLGLCKMISWMYLVGFYSGIFFVMLMSIDRYLAIVHAVFSLRARTLTYGVITSLATWSVAVFASLPGFLFSTCYTERNHTYCKTKYSLNSTTWKVLSSLEINILGLVIPLGIMLFCYSMIIRTLQHCKNEKKNKAVKMIFAVVVLFLGFWTPYNIVLFLETLVELEVLQDCTFERYLDYAIQATETLAFVHCCLNPIIYFFLGEKFRKYILQLFKTCRGLFVLCQYCGLLQIYSADTPSSSYTQSTMDHDLHDAL |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−K5-5 CD_antigen, CD194
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Quick Database Links
UniProt
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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