Human CCR4 Protein, hFc Tag

SKU: orb1173939

Description

Recombinant Human CCR4 with C-terminal human Fc tag

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Key Properties

• Source: Mammalian
• Expression System: HEK293
• Target: CCR4
• Reactivity: Human
• Tag: C-Human Fc Tag
• Molecular Weight: The protein has a predicted molecular mass of 37.1 kDa after removal of the signal peptide.The apparent molecular mass of CCR4-hFc is approximately 35-55 kDa due to glycosylation.
• Expression Region: CCR4(Met1-Glu39)(Ala99-Lys111)(Ser176-Val200)(Val268-Tyr284) hFc(Glu99-Ala330)
• Purification: Purified from cell culture supernatant by affinity chromatography
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Reconstitution: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CC-CKR-4;CD194;ChemR13;CKR4;CMKBR4;HGCN:14099;K5-5

Human NKp30 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7H6, His tagged protein orb757435 can bind Human NKp30, hFc tagged protein (orb689425) in a linear range of 250-2000 ng/ml.

B7H6 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with 2 μg/mL Human NKp30 Protein, hFc Tag (orb689425) on Expi293 cells transfected with human B7H6 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Quick Database Links

Gene Symbol

CCR4

UniProt

P51679