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Catalog Number | orb1743345 |
---|---|
Category | Proteins |
Description | Recombinant Human BACE1 Protein with C-terminal 6×His tag |
Reactivity | Human |
Tag | C-6×His Tag |
Form/Appearance | Liquid, PBS with 0.05% Proclin300, 1% rAlbumin |
Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
MW | The protein has a predicted molecular mass of 49.3 kDa after removal of the signal peptide. The apparent molecular mass of BACE1-His is approximately 55-100 kDa due to glycosylation. |
Target | BACE1 |
UniProt ID | P56817 |
Source | Mammalian |
Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Alternative names | ASP2;BACE;HSPC104 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Flow cytometry analysis with 1 μg/mL PE-conjugated GPRC5D-mFc protein (orb1291784) on Anti GPRC5D CAR-Jurkat cells (Blue histogram) or negative Jurkat cells (Red histogram).
SDS-PAGE: > 95% | |
98.9 kDa (predicted). Due to glycosylation, the protein migrates to 145-150 kDa based on Tris-Bis PAGE result. |
98.00% | |
100.7 kDa (predicted). Due to glycosylation, the protein migrates to 170-200 kDa based on Tris-Bis PAGE result. |
Greater than 90.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
98.00% | |
49.9 kDa (predicted); 60-65 kDa (reducing condition, due to glycosylation) |
> 95%, determined by SDS-PAGE and SEC-HPLC | |
This protein contains (Met 1-Thr 457) of human BACE1 (NP_036236.1) precursor was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |