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Catalog Number | orb753334 |
---|---|
Category | Proteins |
Description | Recombinant human BACE1 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His-IF2DI Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 32.5 KDa |
UniProt ID | P56817 |
Source | E.Coli |
Expression Region | 46-152aa |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH 7.4 |
Alternative names | APP beta-secretase protein, ASP2 protein, Aspartyl Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
98% | |
49.0 kDa | |
Human BACE-1, Tag Free (orb257210) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1). |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.3 kDa after removal of the signal peptide. The apparent molecular mass of BACE1-His is approximately 55-100 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |