Human Apelin (APLN) ELISA Kit

• Catalog Number: orb777933
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin(APLN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin(APLN).
• Reactivity: Human
• Concentration: 1600 pg/mL
• UniProt ID: Q9ULZ1
• Tested applications: ELISA
• Application notes: standard: 1600 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APLN in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 25-1600 pg/mL
• Sensitivity: 8.4 pg/mL
• Sample Types: serum, plasma and other biological fluids
• Alternative names: XNPEP2 ELISA Kit, APEL ELISA Kit, APJ endogenous l
• Note: For research use only