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| Catalog Number | orb777933 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin(APLN) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin(APLN). |
| Tested applications | ELISA |
| Reactivity | Human |
| Range | 25-1600 pg/mL |
| Assay Time | 2.5h |
| Concentration | 1600 pg/mL |
| UniProt ID | Q9ULZ1 |
| Sensitivity | 8.4 pg/mL |
| Alternative names | XNPEP2 ELISA Kit, APEL ELISA Kit, APJ endogenous l Read more... |
| Note | For research use only |
| Application notes | standard: 1600 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APLN in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
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- Item 1 of 1
Human APLN ELISA Kit [orb405633]
Human
125 pg/mL-8000 pg/mL
31.25 pg/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T
