Human Apelin (APLN) ELISA Kit

SKU: orb1807120

Description

Human Apelin (APLN) ELISA Kit

Research Area

Cardiovascular Research

Images & Validation

• Application Notes:
  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human APLN. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human APLN. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: plasma, serum
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 62.5-4000pg/mL
• Sensitivity: 25.2 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APEL, XNPEP2

Standard curve for orb1807120 ELISA kit.

Quick Database Links

Gene Symbol

APLN

UniProt

Q9ULZ1